One-tube test for determining oxidative or fermentative metabolism of bacteria.
نویسنده
چکیده
A one-tube test for distinguishing oxidation from fermentation in the metabolism of carbohydrates was examined on 1,200 gram-negative bacilli. There was a 100% correlation between the one-tube method and the standard two-tube procedure.
منابع مشابه
Modified medium for the oxidation-fermentation test in the identification of marine bacteria.
Using the medium of ZoBell as a basal formulation, we developed a medium to determine the oxidative-fermentative metabolism in marine bacteria. Different oxidative and fermentative collection strains, as well as a group of unidentified marine bacteria, were tested on modified ZoBell medium and other conventional media. In general, superior results were obtained with the modified ZoBell medium s...
متن کاملOne-tube oxidation-fermentation methods: limitations posed by atypical fermentative reactions.
Atypical fermentation in the two-tube method of Hugh and Leifson (1953), whereby acid was produced in the sealed tube but not in the open tube, was determined to be the result of a preferential metabolism of peptone over the reactant carbohydrate. These atypical fermentations are only partially detected by one-tube oxidation-fermentation methods, and as such they constitute a source of limitati...
متن کاملThe Use of Sodium Azide for Determining the Fermentative Ability of Yeast Developed Under Different Oxygen Tensions.
Since the time of Pasteur quantitative differences in the rate of fermentation or respiration have been reported for yeast developed under different oxygen tensions. Fermentative activity in the majority of cases has been based on rates of C02 production, although to a limited extent rates of glucose utilization and the rates of alcohol formation have also been employed. It should be emphasized...
متن کاملEvaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli.
The Oxi/Ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. The nine biochemical reactions used as the initial tests in the Oxi/Ferm tube system showed a close agreement with the same conventional reactions. However, the system was onl...
متن کاملMacromolecular crowding explains overflow metabolism in cells
Overflow metabolism is a metabolic phenotype of cells characterized by mixed oxidative phosphorylation (OxPhos) and fermentative glycolysis in the presence of oxygen. Recently, it was proposed that a combination of a protein allocation constraint and a higher proteome fraction cost of energy generation by OxPhos relative to fermentation form the basis of overflow metabolism in the bacterium, Es...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 27 4 شماره
صفحات -
تاریخ انتشار 1974